WesternBlots - ImmunoBlots                             


Toxoplasma Gondii IgG + IgM + IgA Immunoblot

Immunoblot for the detection of IgG, IgM or IgA antibodies against Toxoplasma gondii in serum


Toxoplasma gondii, which is the only species of the genus Toxoplasma, is an ubiquitary populated, obligatorily endocellular parasite, using many mammals and even birds as an intermediary host. The epidemic attack on the population shows geographic differences and is age-dependant.

Most of the infections with T. gondii develop inapparently, whereas for a lower percentage of infected persons slight symptoms appear (swelling of lymph nodes of the neck, sometimes fever and distinct swellings of the lymph nodes). In cases of acquired immune deficiency (cytostatic remedies, AIDS), a huge multiplication of Toxoplasma in the brain with abscess-like destructions of the brain can appear as well as a systematic dissemination.

If the mother is infected for the first time during pregnancy, the placenta can be affected and a defection of the fetus may occur during the parasitemic stage. The earlier the fetus is affected the more severe are the possible late sequelae such as hydrocephalus, calcification of the brain and chiriotenitic scars, and on the other hand the risk of affection increases with the stage of pregnancy. Especially newborns that are infected late during the pregnancy show only little or no signs of damage, but for a part of these children late sequelae such as chirioretinitis or mental retardation are possible.

The existence of anti Toxoplasma IgM suggests a fresh infection, but is not a proof for it, for under circumstances these IgM are able to persist for years.

Our test is not authorized by the Paul-Ehrlich-Institute and is only applicable for research.


 Antigen: Trachitocytes (purified).

Cutoff - Standard setting: IgM/ IgA - 10 %, IgG - 20 %, freely adjustable as required.

 The following bands are characterized:

16 kD, 27 kD, 30 kD (p30), 35 kD, 40 kD, 60 kD, 67 kD, 70 kD, 88 kD, 94 kD, 110 kD.

Comments: Little has as yet been reported on the importance of the different bands. Only the 30 kD band has been described. Remaining titers, especially IgG, can persist a long time, although p30 is then rarely found. This test kit has not been approved according to AMG.

Intepretation IgG

It is a positive reaction if P 30 (or 35 kD) as well as a further band is detected.

The result is uncertainly positive if only P 30 (or 35 kD) is detected.

Interpretation IgM

It is a positive reaction if P 30 or 35 kD is detected alone.

Finally, it should be mentioned that each single state and also a blot state must always be regarded in combination with further data. A final diagnosis whether there is an infection or not can only be made with all laboratory data and information from the clinic.


IgM - P30 positive suggests a fresh infection. In some cases IgM titres can persist.

IgA - P30 positive in cases of an acute infection.

IgG - P30 and one further band marked = Toxoplasma positive

P30 marked only = Toxoplasma possibly positive.



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